|
|
|
| Registros recuperados: 148.874 | |
|
|
| Fleuri,Luciana Francisco; Sato,Hélia Harumi. |
| Objetivou-se, no presente trabalho, a aplicação de β-1,3 glucanases e quitinases da linhagem Cellulosimicrobium cellulans 191 na lise de leveduras e inibição de fungos, respectivamente. O delineamento experimental mostrou que as melhores condições para a lise de Saccharomyces cerevisiae KL-88 pela β-1,3 glucanase foi pH 6,5 e 35ºC. As células de leveduras incubadas por 10 h em frascos sem agitação mostraram-se mais susceptíveis à lise pela ação da enzima. Foi obtido maior lise da levedura quando a suspensão de células foi submetida ao tratamento com β-1,3 glucanase e cisteína 1mM. A enzima invertase intracelular ou ligada à célula de S. cerevisiae KL-88 e K. marxianus NCYC 587 foi extraída após tratamento da suspensão celular com β-1,3 glucanase, sendo que... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-1; 3 glucanases quitinases lise celular inibição. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000400029 |
| |
|
|
| Bertoldo, Edson; Mazaro, Sérgio Miguel. |
| As β-1,3 glucanases de plantas são enzimas abundantes e comumente encontradas no reino vegetal, estando envolvidas em vários processos fisiológicos, especialmente na defesa vegetal. A defesa vegetal é baseada em uma série de barreiras pré e pós-formadas, ambas subdivididas em estruturais e bioquímicas. As barreiras estruturais atuam contendo o patógeno fisicamente, enquanto a ação bioquímica opera através da produção de substâncias tóxicas e antimicrobianas, como a produção de enzimas hidrolíticas (proteínas relacionadas à patogênese ou PR proteínas). O objetivo desta revisão é abordar o papel das β-1,3 glucanases em plantas, sobretudo nos mecanismos de defesa vegetal ativados pela indução de resistência, a qual tem por objetivo estimular os vegetais a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proteínas-PR; Indução de resistência; Fitopatógenos; Hidrólise. |
| Ano: 2018 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/17979 |
| |
|
| |
|
|
| Otaviano,Antonio R.; Lima,André Luis F.; Laureano,Monyka M.M.; Sena,Janete A.D.; Albuquerque,Lúcia G. de; Tonhati,Humberto. |
| Mozzarella cheese is traditionally prepared from bubaline (Bubalus bubalis) milk, but product adulteration occurs mainly by addition of or full substitution by bovine milk. The aim of this study was to show the usefulnes of molecular markers to identify the admixture of bovine milk to bubaline milk during the manufacturing process of mozzarella cheese. Samples of mozzarella cheese were produced by adding seven different concentrations of bovine milk: 0%, 1%, 2%, 5%, 8%, 12% and 100%. DNA extracted from somatic cells found in cheese were submitted to PCR-RFLP analysis of casein genes: α-s1-CN - CSN1S1 that encompasses 954 bp from exon VII to intron IX (Alu I and Hinf I), β-CN - CSN2 including 495 bp of exon VII (Hae III and Hinf I), and κ-CN - CSN3,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Casein; Gene polymorphism; Mozarella; Cheese authentication; PCR-RFLP. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500017 |
| |
|
| |
|
| |
|
| |
|
|
| Silva,Wellington dos Santos; Klautau-Guimarães,Maria de Nazaré; Grisolia,Cesar Koppe. |
| Five restriction site polymorphisms in the β-globin gene cluster (HincII-5'ε, HindIII-Gγ, HindIII-ªγ, HincII-'ψβ1 and HincII-3''ψβ1) were analyzed in three populations (n = 114) from Reconcavo Baiano, State of Bahia, Brazil. The groups included two urban populations from the towns of Cachoeira and Maragojipe and one rural Afro-descendant population, known as the "quilombo community", from Cachoeira municipality. The number of haplotypes found in the populations ranged from 10 to 13, which indicated higher diversity than in the parental populations. The haplotypes 2 (+----),3(----+),4(-+--+)and6(-++-+)onthe βA chromosomes were the most common, and two haplotypes, 9 (-++++)and 14 (++--+), were found exclusively in the Maragojipe population. The other... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Afro-descendant; Β-globin; Haplotypes; Hemoglobinopathies. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300002 |
| |
|
|
| Brazil,Crislane; Oliveira,Débora Francielly de; Duarte,Rafael Augusto; Galo,Juliana Minardi; Lucchetta,Luciano; Santos,Eder da Costa dos; Hashimoto,Elisabete Hiromi. |
| Abstract The β-Glucans content has straight influence on the quality of malt and beer, mainly during the filtration step. Barley presenting high β-Glucan content demands longer germination time at malting. The application of commercial β-Glucanase is an alternative to accelerate the process and preserve the quality of malt. This work aimed to evaluate the effect of commercial β-Glucanase addition in malt produced within reduced germination time (64 h). Micro-malting was conducted with BRS-Caue and Elis barley cultivars at germination time 64 h and 96 h. The β-Glucanase concentration applied were 0, 25, 50 and 100 mg.kg-1. Barley, malt and wort samples were analyzed to check their physical-chemical features. Beers were produced with BRS-Caue malt and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-glucans; Germination time; Commercial enzyme. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100222 |
| |
|
|
| Vasques,Marcia Regina G; Bello,Alexandre Ribeiro; Lamas,Cristiane da Cruz; Correa,Juarez; Pereira,José Augusto Adler. |
| There is a high incidence of infections caused by betalactamase-producing Gram-negative microorganisms in Brazil. These organisms are of clinical and epidemiological importance, since their mobile genetic elements facilitate cross-infection. The present study was conducted in sentinel rectal swabs from patients admitted to a cardiac surgery hospital in Rio de Janeiro, from January through December 2007, in a consecutive manner. The aim of the study was to characterize the genotype and phenotype of these isolates from colonized patients. Biochemical tests, antimicrobial susceptibility tests, a confirmatory test for the expression of extended spectrum betalactamase (ESBL) production and polymerase chain reaction for the blaTEM, blaSHV, CTX-M1, Toho-1 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymerase chain reaction; Infection control; Enterobacteriaceae infections. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100006 |
| |
|
|
| Leite,Clariana Akemi Kariya; Oizumi,Karina Yoshimi; Caleffi-Ferracioli,Katiany Rizzieri; Scodro,Regiane Bertin de Lima; Pádua,Rubia Andreia Falleiros de; Cardoso,Rosilene Fressatti; Pires,Claudia Terencio Agostinho; Siqueira,Vera Lucia Dias. |
| ABSTRACT The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterobacteriaceae; Pseudomonas aeruginosa; Acinetobacter spp; Antimicrobial resistance; Beta-lactamases; Bacterial typing; Intensive Care Unit/study/Brazil; Drug-resistant bacteria.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200609 |
| |
|
| |
|
| |
|
|
| Oliveira,Karen Maciel de; Binda,Nancy Scardua; Lavor,Mário Sérgio Lima; Silva,Carla Maria Osório; Rosado,Isabel Rodrigues; Taguchi,Tatiana Malagoli; Alves,Endrigo Gabellini Leonel; Melo,Marília Martins; Gomez,Marcus Vinícius; Melo,Eliane Gonçalves de. |
| This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MVIIA; Cone snail; Cell viability; Glutamate; Reactive oxygen species; Lipid peroxidation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150 |
| |
| Registros recuperados: 148.874 | |
|
|
|
